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When updating the symbology of a feature service, and overwriting the service using ArcMap, updates made to the symbology are not reflected in a web map after republishing to ArcGIS Online.
This is expected behavior when overwriting a feature service in a web map that already has symbology applied and saved. By design, when the feature service is overwritten, the symbology saved previously in the web map is maintained because overwriting the service only updates the feature service, and not the JSON file of the web map, which stores all properties of the saved web map. The properties of the web map stored in the JSON file include web map layers, labels, pop-up configurations, and symbology.
When overwriting a feature service in the web map, the updated symbology is only reflected in the web map if no symbology is configured and saved in the ArcGIS Online web map viewer prior to overwriting the feature service.
As a workaround, follow the steps provided below: