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After following the steps in the help document below, users and roles are not seen in ArcGIS Server Manager or the Administrators page.
Using a 10.0 .NET SQL Server security store in 10.1
At the release of ArcGIS for Server 10.1 Service Pack 0, there were bugs logged when attempting to use a 10.0 .NET SQL Server security store.
NIM081225 - When using the 10.0 SQL store as the identity store, the users page in Manager hangs indefinitely.
NIM080836 - Attempting to use .NET providers for the user\role store, returns the error "The connection name [string name] was not found in the application's configuration or the connection string is empty".
Install and upgrade to ArcGIS for Server 10.1 Service Pack 1.
ArcGIS 10.1 Service Pack 1 (Desktop, Engine, Server) Announcement