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Adding/deleting rasters in the mosaic dataset isn't reflected in the published image service.
After updating a GIS resource for a service, such as an MXD or raster dataset, the changes are not reflected in the service. Almost all types of ArcGIS Server web services reference a GIS resource. For example, map services reference an MXD, and an image service references a mosaic dataset. By adding or deleting rasters, you essentially modify the GIS resource (a mosaic dataset), and restarting or overwriting the service is necessary after updating settings in the underlying GIS resource.
Re-start the image service after adding/deleting rasters in the referenced mosaic dataset.