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Why does my data frame not align properly when in Layout view?
The reason for this is that you have chosen to display the raster resolution values in the Table of Contents (TOC) for at least one of your raster layers. This is a current issue in ArcGIS that results in the data frame not aligning properly. To remedy this situation, uncheck the 'Display raster resolution in the table of contents' option in the Display tab of the raster layer Properties dialog.